Senescence in cultured trabecular meshwork cells
نویسندگان
چکیده
منابع مشابه
Identification of Kir2.1 channel activity in cultured trabecular meshwork cells.
PURPOSE To study the presence of inwardly rectifying K(+) (Kir) channels in cultured bovine (BTM) and human (HTM) trabecular meshwork cells. METHODS Cultures of BTM and HTM cells were obtained by an extracellular matrix digestion technique. Whole-cell patch-clamp recordings of BTM cells were performed with the appropriate solutions to detect K(+) currents. Also, Western blot analysis of Kir2....
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PURPOSE To determine the effect of the serine-threonine kinase inhibitor H-7, which blocks actomyosin contractility and increases outflow facility in live monkeys, on morphology, cytoskeleton, and cellular adhesions of human trabecular meshwork (HTM) cells in culture. METHODS Cultured HTM cells were videographically recorded and evaluated before and after exposure to H-7 at different concentr...
متن کاملCultured human trabecular meshwork cells express functional growth factor receptors.
PURPOSE To compare the mRNA expression of growth factor receptors in cultured human trabecular meshwork (HTM) cells with ex vivo HTM tissues and to determine whether HTM cells generate a physiologic response after exposure to exogenous growth factors. METHODS The reverse transcription-polymerase chain reaction (RT-PCR) method was used to detect the expression of various growth factor receptor...
متن کاملStromelysin gene transfer into cultured human trabecular cells and rat trabecular meshwork in vivo.
PURPOSE To determine whether stromelysin gene can be introduced into and expressed in the cultured human trabecular cells as well as in the rat eye in vivo through means of a recombinant replication-deficient adenovirus. METHODS Stromelysin cDNA was obtained by reverse transcription-polymerase chain reaction with mRNA extracted from the cultured human trabecular cells after induction with int...
متن کاملsCD44 internalization in human trabecular meshwork cells.
PURPOSE To determine whether soluble CD44 (sCD44), a likely biomarker of primary open-angle glaucoma (POAG), is internalized in cultured human trabecular meshwork (TM) cells and trafficked to mitochondria. METHODS In vitro, 32-kD sCD44 was isolated from human sera, biotinylated, and dephosphorylated. TM cells were incubated for 1 hour at 4°C with biotinylated albumin (b-albumin), biotin-label...
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ژورنال
عنوان ژورنال: British Journal of Ophthalmology
سال: 2007
ISSN: 0007-1161
DOI: 10.1136/bjo.2006.108423